通道校正默认值
Contents
In the course of this example, we will:
| • | Learn how to change channel calibration default settings for FCS Express plots. |
As previously mentioned, the default setting is for FCS Express to use channel calibration on all plots.However, you have the option of turning the channel calibration option off, or on, for a specific plot type.We will now show how you can turn the channel calibration option off for all 2D dot plots.
| 1. | SelectFile Tab→Options. |
TheFCS Express User Optionsdialog now appears (Figure T23.12).
| 2. | Select thePlots→Dot Options→Binned Dots Specific Optionssubcategory on the left side of theFCS Express User Optionsdialog, shown highlighted in grey in Figure T23.12. |
| 3. | Uncheck theUse channel calibration on axesbox, indicated by the cursor in Figure T23.12. |
| 4. | ClickOKto close theFCS Express User Optionsdialog. |
Figure T23.12 FCS Express User Options Dialog -- Unselecting Use channel calibration on axes for 2D Dot Plots
This setting will now ensure that all new 2D dot plots created will not use channel calibration.If we would like to remove the channel calibration for other types of 2D plots, we would need to go to the specific subcategory for that type of 2D plot under thePlotscategory on theFCS Express User Optionsdialog.
Note that you always have the option of setting or removing the channel calibration for a specific plot, rather than for all plots, using either theFormat→Plot Options→Specific Optionscommand, or using theFormatdialog from the plot pop-up menu as shown in the previous steps.When theFormattingdialog associated with the plot appears, remember to selectDot Options→Binned Dot Specific Optionsfor 2D dot plots orHistogram→Histogram Specific Optionsfor histograms.Then, select or unselect theUse channel calibration on axesoption.
Remember, it is important to uncheck theUse channel calibration on axesoption on any histograms you are going to use to set up a new bead channel calibration.