使用DNS-IJXwriter Excel宏,来创建一分析配置文件

在这一节中,我们将学习如何使用DNS-IJXwriter Excel宏,来自动生成一.ijx分析配置文件。此DNS-IJXwriter Excel宏是包含一个宏的Excel文件,由Peter Haub编写,它会自动从图像的文件夹中读取文件名,然后生产一.ijx文件。

 

1.请打开位于教程样本数据库中ImageJ教程ImageJ DNS Macros文件夹
2.点击Enable Content(启用内容)按钮(图T28.102)。

 

Figure T28.102 Enabling macros in a Microsoft Excel file.

Figure T28.102 Enabling macros in a Microsoft Excel file.

 

 

3.点击Read File Names(读取文件名)命令(图T28.103)。

 

Figure T28.103 Clicking on the Read File Names macro button

Figure T28.103 Clicking on the Read File Names macro button

 

4.请导航到 Tutorial Sample Data Archive(样本教程数据库) 中ImageJ教程文件夹下的"Mutli Channel Image Experiment(多通道图像实验)"文件夹。
5. 点击OK(图T28.104)。        

 

Figure T28.104 Navigating to the ImageJ Tutorial folder within the Tutorial Sample Data Archive

Figure T28.104 Navigating to the ImageJ Tutorial folder within the Tutorial Sample Data Archive

 

 

6.在电子表格的“Channel”列(D列),为每个图片输入相应的通道名称(即"DAPI"或者"FITC")。 现在,您的电子表格看上去应如图T28.105。

 

Figure T28.105 DNS-IJXwriter.xlsm Excel file after file names have been read and channel names entered into the appropriate column.

Figure T28.105 DNS-IJXwriter.xlsm Excel file after file names have been read and channel names entered into the appropriate column.

 

 

7. 点击Write IJX File(写入IJX文件)命令(图T28.105,红框显示)。

 

"DeNovo.IJX"这个分析配置文件,就被写入到TutorialSampleData/ImageJ Tutorial/Multi Channel Image Experiment(多通道图像实验)目录文件夹。此.ijx文件,现在可能使用DNS MacroExample.ijm宏,在ImageJ中进行了加工处理,为了使该文件变为FCS Express可读的.ijout文件。