光谱图像可以被叠加(例如图28。8)在FCS Express中，可以使用相同的方法叠加直方图到其它图像上。要了解更多关于数据叠加的生成，请参考Formatting 1D Spectrum Plots(一维光谱图的生成)中的副标题叠加。

Figure 28.8 - Spectral plot with two overlays from different spectral datafiles, each with a different gate applied.

Figure 28.8 - Spectral plot with two overlays from different spectral datafiles, each with a different gate applied.

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