The following tips and instructions will aid in importing and using Accuri CFlow experiments:

 

Recommended settings in FCS Express to help improve performance when importing BD Accuri C6 experiments.

 

1.Open theOptionsdialog using theFile tab→Optionscommand.

 

2.Select theData Loading→FCS File Optionscategory.Make sure thatAllow Multiple Datasetsis unchecked.Although this setting is not necessary for loading a C6 data file, it will allow you to add files to the data list, which is necessary for batch processing.

     

 

Loading BD Accuri C6 software data into FCS Express plots.

 

1. Insert a plotfrom the Insert Tab.When the Select a Data File dialog opens, change the file type toC6 files (*.c6; *.cfl; *.ci).

 

2.Navigate to the BD Accuri C6 software file that you want to open, and double-click on it.The dialog will then display all of the fcs files contained inside the C6 file.Select the one that you are looking for.

     

3.PressOpen.