Applying Compensation Definitions
Contents
Every overlay on a plot, and every file loaded as a plate, can have a different compensation definition. To change the compensation for an overlay, right-click on the plot and select Format from the pop-up menu. Go to the Overlays category and select the compensation definition you want to use. Alternatively, you can use the Format tab→Plot Options→Overlays command.
There are also many drag and drop commands for compensations. You can drag and drop compensations from the Compensations navigator to any plot, any layout, and from one matrix to another to apply and edit compensations (Figure 16.18).
To apply a compensation definition by dragging and dropping from the Compensations navigator follow the steps below.
| 1. | Left click and hold on a compensation. |
| 2. | Drag the compensation to the intended plot (red arrow). Blue borders around the plot will indicate you can drop the compensation. |
| 3. | Drop the compensation by releasing the left mouse button. |
The compensation will then be applied to the plot (green arrow).
Figure 16.18 Dragging and dropping a compensation from the Compensations navigator onto a plot to apply the compensation.
Compensations may be dragged and dropped between different layouts by dragging the compensation definition from the Compensations navigator in one layout to the Compensations navigator or on a plot in another layout much like Steps 1 - 3 above. This will allow you to easily apply compensations between different layouts and experiments.
A compensation definition may also be dragged and dropped onto another compensation definition. This allows you to easily copy matrices from one compensation definition to another.
You can set a compensation as default for the layout, so it will automatically apply to all experimental files in your Data List as you click Next/Prev or perform a Batch Process. See the Automatic Compensation and Batch Processing section of the relevant tutorial.
You can save compensation definitions to folders, and have them automatically apply to the FCS files in the folder.
Additionally, standard copy and paste commands may be used to move matrices from one compensation definition to another within the Compensations navigator.
Note that various other functions in FCS Express allow you to apply a compensation definition. For instance, when performing Parameter Math you can choose whether a compensation definition is applied prior to performing the math.