In high-throughput laboratories, flow cytometry and cell imaging are often done in 96-well microtiter plates. The data from a 96-well microtiter plate normally contains 96 separate data files. FCS Express can automatically merge the files into one dataset and associate each "cell" with the particular well that it was located in.

 

A Heat Map is a graphical representation of your data in a plate-shaped grid with each well of the plate color-coded based on the statistic being displayed. By choosing an appropriate color scheme and statistic to display, you can get an overview of all of the wells in your experiment. This enables rapid visualization of outliers by comparing the statistical data in an individual well to the statistical data of the entire microtiter plate. You can also create Well Gates to conveniently pool your data.  Note that the heat map feature is an add-on that must be purchased.

 

 

Picture Plots are two-dimensional displays of the morphology of cells (or other objects). Think of them as gated collections of cell images. The location of individual cells is shown along the x- and y-axes of the plot. Gates can be set on individual cells or groups of cells.