The following tips and instructions will aid in importing and using Accuri CFlow experiments:

 

•Recommended settings in FCS Express to help improve performance when importing BD Accuri C6 experiments.

 

1. Open the Options dialog using the File tab→Options command.

 

2. Select the Data Loading→FCS File Options category. Make sure that Allow Multiple Datasets is unchecked. Although this setting is not necessary for loading a C6 data file, it will allow you to add files to the data list, which is necessary for batch processing.

 

3. Uncheck Validate that files are FCS Files before displaying them in a file dialog. This change, while not necessary for loading a C6 data file, will speed up adding files into the file list.

     

 

•Loading CFlow data into FCS Express plots.

 

1. Insert a plot from the Insert Tab. When the Select a Data File dialog opens, change the file type to C6 files (*.c6; *.cfl).

 

2. Navigate to the CFlow file that you want to open, and double-click on it. The dialog will then display all of the fcs files contained inside the C6 file. Select the one that you are looking for.

     

3. Press Open.

www.denovosoftware.com