Adding a Combination Gate Matrix
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Adding a Combination Gate Matrix
A combination gate matrix is a set of combination gates, created from one or more previously existing gates. This set of gates contains every possible positive and negative value for the previously existing gates. For example, if we start with a gate labeled "CD4", and another gate labeled "CD25", select both gates, and create a combination gate matrix from them, four new gates will be created (Figure 9.24):
• CD4+/CD25+
• CD4-/CD25+
• CD4+/CD25-
• CD4-/CD25-
Figure 9.24 Combination Gate Matrix Example. Left: Gates applied to select all CD4+ or CD25+ cells. Right: Combination Gate Matrix applied to create gates for all four combinations.
Once the new gates are created, they are treated as normal gates. Any changes to the original gates (such as renaming) will not cause the new gates to be renamed. The new gates can also be deleted or edited like any other combination gate.
To add the combination gate matrix:
After inserting a Gate View object onto the layout (Insert→General→Gate View), select the gates that you want to use via SHIFT-click or Ctrl-click, right-click on the Gate View, and select Add Combination Gate Matrix from the pop-up menu (Figure 9.25).
Figure 9.25 Adding a Combination Gate Matrix
If you selected three gates to create the matrix then, after the new matrix has been added, eight new gates will have been created. The gate will now look like Figure 9.26.
Figure 9.26 After Adding a Combination Gate Matrix