In addition to the formatting options common to all Objects, and those common to all Plots, single parameter histograms have certain properties, such as overlays, markers, magnification, and other specific options which can be formatted.

 

Overlays are defined in FCS Express as a data set that is displayed on a plot. A data set for a histogram consists of a data file and parameter combination. A histogram must contain one or more overlays. Overlays can be added or deleted from a histogram. Furthermore, while any number of data files with parameters different from the first overlay (also known as the base overlay) can be added to the same histogram, the axis title and scaling will always be taken from the base overlay.  The histogram legend is valuable for displaying and organizing multiple overlays.

 

Markers are regions defined along a single parameter.

 

Magnification of a histogram allows you to reset the minimum and maximum axis scale and resolution to restrict your view to a certain region of the histogram.

 

The histogram specific options include normalization, plot resolution, presentation choices for multiple overlays, and the choice of a control histogram for histogram subtraction statistics.

 

You can always format a histogram by right-clicking on the plot, selecting Format from the pop-up menu, and choosing a category. This method will allow you to use the Preview button to see what the changes would look like without actually accepting the changes. Alternatively, once the histogram is selected, you can choose the desired category from the Format tab of the Ribbon.