FCS Express comes with a free version of Multicycle capable of performing a 1-cycle fit with no background correction. The full version of Multicycle may be purchased as an add-on for FCS Express from De Novo Software, or as standalone software from Phoenix Flow Systems (http://www.phoenixflow.com).

 

The Multicycle model has been described extensively in the following publications:

 

•Rabinovitch, P.S. DNA content histogram and cell-cycle analysis. Methods in Cell Biology. 41:263-296, 1994.

 

•Rabinovitch, P.S. Practical considerations for DNA content and cell cycle analysis. In: Clinical Flow Cytometry: Principles and Applications (Bauer, K.D., Duque, R.E., and Shankey, T.V., eds.) Williams and Wilkins, Baltimore, pp. 117-142, 1992.

 

•Rabinovitch, P.S. DNA Histogram Analysis. In: Cell Growth and Division, 2nd Edition. (G. Studinski, ed.). Oxford University Press, Oxford, 1996.

 

•Kallioniemi, O-P., Visakorpi, T., Holli, K., Isola, J.J., and Rabinovitch, P.S. Automated peak detection and cell cycle analysis of flow cytometric histograms. Cytometry 16:250-255, 1994.