Assigning a gate to be Data Specific is accomplished from the Gating tab through the steps below:
1. Click on the gate to convert to a data specific gate (Figure 9.10 - Number 1) on a plot.
2. Click on the Gating tab of the Ribbon.
3. Select Create Gates→Data Specific Gates (Figure below - Number 2).
Figure 9.10- Data File Specific gates are created using the Data Specific Gate button from the Gating tab > Create Gates section.
The Gate Navigator will now reflect the presence of a Data Specific Gate for File 4 (ApoMono.004 compensated) (Figure 9.11).
Figure 9.11 - The gate navigator before and after creating a data file specific gate on File 4 (ApoMono.004).
The shape and position of Gate 1 on File 4 can now be modified independently from the master gate (File 1 through File 3) Figure 9.12.
Figure 9.12 - An example of a data file specific gate for Gate 1 that has been made Data File Specific for File 4.
A Data Specific Gate can be reverted back to resemble the Master gate (i.e. to loose its data specificity) in one of three ways:
Option 1:
1. Click on the gate that need to be reverted to Master gate (Figure 9.13 - Number 1).
2. Click on the Gating tab of the Ribbon.
3. Select Create Gates→Data Specific Gates (Figure below - Number 2).
Figure 13 - DSG can be deleted using the Data Specific Gate button from the Gating > Create Gates ribbon bar.
Option 2:
1. Select the Data Specific Gate for the File in the Gate Navigator.
2. Press Delete.
Option 3:
4. Right click on the Data Specific Gate in the Gate Navigator.
5. Select Delete Data Specific Gate(s)(Figure 9.14).
Figure 9.14- A Data File Specific Gate can be removed using the right click menu in the Gate Navigator.
When a Data Specific Gate is deleted the Master Gate will be used on any files that had used the Data File Specific gate.
Promoting a Data Specific Gate to the Master Gate
A Data Specific Gate can be promoted to be the Master gate within the Gate Navigator:
1. Right click on the Data Specific Gate in the Gate Navigator.
2. Select Promote Data Specific Gate to Master from the right click menu (Figure 9.15).
(Figure 9.15) - Selecting promote data specific gate to master gate in the gate navigator.
When promoting a Data Specific Gate to Master Gate:
•All of the files that used the original Master Gate will use the Data Specific Gate.
•The original Data Specific Gate is removed from the Gate Navigator (Figure 9.16) - left).
Figure 9.16 - A data specifc gate was promoted to a new master gate. Note the original data specific gate is no longer present.