You can set a gate on one or more wells in a heat map. The wells need not be contiguous.  To create a Well gate:

 

1. Select the Gating tab→Create Gates→Well command. (Alternatively, right-click on the heat map and choose Create gate→Well).

2. Click anywhere on the heat map. An Editing New Well Gate dialog (with instructions) for the well gate will appear above the heat map (Figure 32.11).

 

 

 

To set the specific gate do one or more of the following:

 

•Click on an individual well to select it as part of the gate.

•While holding the Ctrl key, click on multiple wells.

•Click and drag a rectangle across multiple wells to select all wells within the rectangle as part of the gate.

 

The Create New Gate dialog will appear. Choose a name and color, and click on OK.

 

The well(s) in the gate will be outlined by a circle that is the same as the gate color (e.g., the wells outlined in light blue in Figure 32.12).

 

 

 

To change the gate you just created, click or click-drag on new well(s). The original well(s) will no longer be part of the gate.

 

To extend the gate you just created to additional wells, hold down the Ctrl key while clicking on individual wells (or while dragging a rectangle across a group of cells). The new well(s) will be added to the original well(s) in the gate.  Notice a non-contiguous region was selected (Figure 32.13).

 

 

 

 

Click Close when you are finished editing.

 

To activate (and modify) an existing Well gate, right-click on the Heat Map, select Edit gate, and choose the gate to activate from the submenu.