Many flow cytometers are capable of recording a time parameter in the .fcs file, allowing for kinetics experiments to be performed. Kinetics experiments are particularly useful for examining changes in DNA content, antibody binding, and calcium flux over time. By adding a compound (usually a drug) to a sample and monitoring influx, efflux, or binding of your probe over time, unique information can be gathered. FCS Express offers the capability to display and analyze kinetics data in an easy and user friendly manner while updating your plots as your analysis changes.

In the course of this tutorial we will show how to:

•Create parameter math formulas to view ratios between two parameters.

•Create gates and display statistics for Kinetics Plots.

•Plot Mean, Geometric Mean, Median, % Above Threshold, and Count.

•Overlay multiple Kinetics Plots.

•Format a Kinetics Plot to view the data at the correct resolution.

 

In the first exercise, we will create parameter math formulas for use in kinetics plots.