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Selecting the Default Compensation

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FCS Express has many default options for compensation definitions when opening files or adding overlays to a plot. These options are found on the General page of the Options dialog (File tab→Options) in the Default Compensation to Use drop-down list. The options are described in the table below.

Option	Explanation
None	When adding a new plot, no compensation will be used. When changing a file on an existing plot, the compensation that was applied to the plot before the change will continue to be used.
Compensation Default	The compensation definition that was manually Set as Default will be used. To set the definition, see Setting Up Compensation Definitions.
FCS File	The compensation definition stored with the file is automatically used when the data file is displayed on a plot. This is commonly the matrix defined at the time of acquisition (for FCS 3.0 or later format files), or it may be a compensation that was Assigned to an .fcs file by FCS Express.
FCS File, then Compensation Default	See FCS File, above. However, if there is no definition, the default compensation definition will be applied (see Setting Up Compensation Definitions).
Compensation Default, then FCS File	See compensation default above. However, if there is no Compensation Default, the compensation stored with the file will be automatically loaded as described in FCS File above.