Sometimes, you need to know the absolute concentration of the cells in your sample. For example, you may be determining absolute lymphocyte counts or counting bacterial populations. While most instruments allow for the accurate determination of counts, they often do not report the volume of the sample that was measured to obtain those counts.

 

FCS Express can calculate the sample volume from the flow rate and the sample acquisition time, or from counts of beads added to a sample at a known concentration.  It can also read the $VOL in FCS 3.1 files or the equivalent value from Amnis DAF files.

 

FCS Express allows you to:

 

•Calculate individual concentrations.

 

•Have the concentration calculated automatically and appear directly on the statistics window.