The Gates to Display formatting options are for setting which gates to use to color events. Gate display functions are available for dot plots, color dot plots, and scatter plots. You can also choose which gates to display in a data grid.
You can edit the Gates to Display in one of two ways:
•Select the plot(s) and use the Format→Data Options→Gates to Display command (Figure 8.107).
•Right-click on the plot, select Format from the pop-up menu, and choose the Display Gates Options category (located under the Plot Options category for each specific type of plot).
Figure 8.107 Formatting Gates to Display dialog
If you have one overlay on a dot plot, the dots are shown in the gate color. If you have more than one overlay, the colors will reflect the different overlays, which are specified in the Overlays section of the Format window.
By default, the All Gates option is selected. However, you can select Only the gates checked below and then select the gates whose colors you wish to display. Alternatively, select All gates except those checked below and then choose the gates whose colors you do not wish to display.
You can also choose how to display the gate color when a new gate is created on the layout. By choosing either the second or third radio buttons in the When a new gate is created on the layout category, you can have FCS Express display the gate color on a back-gated plot if the new gate was created on a plot showing the same data file, or only on a plot showing the same parameters (as the plot where the new gate was created).