Results from a SPADE transformation are not saved within the layout or data file but are instead calculated automatically (or manually) and applied to plots/files when the layout is reopened. When working with large data sets or computationally intensive transformations the team at De Novo Software recommends saving/exporting SPADE results in a new file so you may more easily work with the data set without having to recalculate the SPADE transformation. SPADE result are not compatible with the Flow Cytometry Standard (i.e. FCS) file format and need to be saved as De Novo Software data Stream (i.e. DNS) file format.

 

Exporting/Saving SPADE results as part of a new .DNS file is easily accomplished by:

 

1. Apply the SPADE transformation of interest to a given plot.

2. Click on the plot to select it.

3. Click on the Data tab→Save/Load→Export from the ribbon to launch the Export Data dialog.

4. Select the additional SPADE Cluster ID parameter from the list of Parameters to Export.

5. Perform the export normally according to the full instructions at Exporting FCS File Data from a Single File, being sure to select DNS data Stream file as file type for the output file.

 

It is also recommend that the layout in which the transformation was create is saved so you may review the settings used to create the SPADE transformation at anytime in the future.