Software Compensation Caveats

Software compensation is not a panacea for all compensation problems. Software compensation works best on high-resolution (> 1024 channel) linear data. Compensation must be performed on linear data. Therefore, data that is stored in logarithmic format (as is sometimes the case with FCS 2.0 data files) must be converted to linear prior to compensation. This conversion from logarithmic to linear can induce rounding errors. In addition, data that is stored as less than 1024 channels is rarely compensated accurately with software. However, the majority of data stored as 1024 channels or greater can be software compensated.

 

Additionally, certain file formats supported by FCS Express do not support storing compensation matrices separately from the data file and the these data sets will only display and utilize the raw data values, compensated values, from the time of acquisition. Examples include .csv files and .MQD (MacsQuant) files.

 

For a detailed description of how software compensation is implemented in FCS Express, please see our Frequently Asked Questions at http://www.denovosoftware.com/site/compensationdetails.shtml.