Concentration Calculator ($VOL keyword)
Contents
In the course of this example, we will:
•Set up the concentration calculator using the volume ($VOL) keyword in the data.
•Obtain a one time result.
| (Note that the following sections build upon steps 1 through 7 in the Setting up the Beads Gates chapter) |
| 1. Select the Tools→Calculate→Concentration Calculator command. |
The Concentration Calculator dialog appears again as shown in Figure T24.5.
2.Select the File to analyze, in this case Monocytes.002, from the drop-down list (Figure T24.5, 
).
3.Select Monocytes as the Gate to analyze, from the drop-down list (Figure T24.5, 
).
4.Select the Volume keyword in the data radio button to specify the Volume Calculation type (Figure T24.5, 
).
5.Click the Calculate button.
In the Results section of the Concentration Calculator, the volume of the sample analyzed appears in the Volume Analyzed (Figure T24.5, 
) field and the concentration of the cells in the Monocytes gate appears in the Sample Concentration 
field (Figure T24.5, ).
Figure T24.5 Concentration Calculator Dialog Using the $VOL keyword
8.Click Cancel to close the Concentration Calculator dialog.
Note: The volume and concentration values may differ due to gate placements. Minor variances in the numerical values that are represented in the tutorial images are expected. Please save this layout to your desktop if unable to complete the next tutorial section.
Next, we will show the calculated concentration in a statistics window.