In the course of this example, we will:

•Learn how to save and load channel calibration sets.

Now that you have set up this calibration (or in the future, multiple calibration sets), you can save them in order to use them for other layouts. For example, if you acquired multiple samples which you are analyzing in separate layouts, you can set up the calibration in one layout, save it, and then load it in all the other layouts. That way you avoid the need to repeat the calibration process in every layout. Bear in mind that the calibration is applicable only to the samples acquired during the same experimental run using the exact same cytometer setup.

 

We will proceed to save the calibration setup using the layout from the previous section.

1.Select the Tools tab→Calculate group→Channel Calibration command (Figure T25.5).

The Channel Calibration dialog will appear as previously shown in Figure T25.8.

2.Select the calibration you created. There should only be one.

3.Click the Save button.

The Save As dialog appears.

4.Navigate to the Tutorial Sample Data folder and enter "CalibDemo".

5.Click Save.

We now will load the calibration setup into a new layout.

6.Click on Cancel to close the Channel Calibration dialog.

7.Select Save icon in the upper left of window to save your current layout.

8.Key Ctrl+N on the keyboard, to open a new blank layout.

9.Select the Tools tab→Calculate group→Channel Calibration command.

The Channel Calibration dialog appears empty, with no calibrations defined, as previously shown in Figure T25.6.

10. Click the Load button.

The Open dialog appears where we can select the calibration file to load.

11. Navigate to the Tutorials Sample Data folder and select the "CalibDemo" file that you saved from steps 1 through 5 above.

12. Click Open.

The Channel Calibration dialog will resemble Figure T25.8, with the same calibration information you saved in the other layout.

13. Click on Cancel to close the Channel Calibration dialog.

14. Close the current blank layout to return to the original layout we were working on before.

Finally, we will set up channel calibration defaults.