FCS File Options

Text to display in selection dialog

FCS Express allows you to display keyword values (e.g., the SAMPLE ID) as well as the file name. For a list of keywords, use the Data Info View Header command, choose an FCS file, and click on the All Keywords… button.

Choose The text below from the drop-down list to unlock the edit field below. Enter the keyword(s) you wish to view using < and > to bracket the keyword. For example, <$SAMPLE ID> will display the Sample ID as the file name.

Dataset Name Keyword

Select the keyword to use as the default dataset name when selecting from multiple datasets.

Allow Multiple Datasets

Some FCS files contain multiple data sets. If this option is checked, you can select one of the embedded datasets.

Default Dataset to use

This option specifies which dataset should be loaded by default when Allow Multiple Datasets is not checked. In that case, when you select a file in the dialog that has multiple datasets, will load the dataset number corresponding to the value selected instead of displaying a list of datasets. If the dataset does not exist, FCS Express will load the first dataset in the file.

FCS v3.0 $TIMESTEP units

Choose a unit to use when displaying a Time parameter in FCS Express from the drop-down list. The Default time option leaves the time parameter at the same raw value as is stored in the FCS file. Choosing a unit from the drop-down list will use the $TIMESTEP keyword to convert the raw value to the specified unit from the list.

Validate files are .fcs files before displaying them in a file dialog

If this option is checked, FCS Express will perform a validation before displaying the files in a selection dialog. Unchecking this option may speed up file selection.

Use the FCS File icon for FCS Files, regardless of the file extension

FCS files may not be named with consistent extensions. If this option is checked, FCS Express will check each file to determine if it is an FCS file (irrespective of its extension) and display FCS Files with a consistent icon. Note: This option may slow down the file dialog display.

Keyword To Use as Parameter Name

Choose a keyword (or combination of keywords) to use as parameter names from the drop-down list.

Allow opening BD FACSDiva data files that were generated by exporting a FACSDiva Experiment

Check this box to open numerically-named files exported from BD FACSDiva. See http://www.denovosoftware.com/site/kb-invalidfilefromfacsdiva.shtml for a detailed explanation. Re-exporting the files is a better option if you have access to FACSDiva and the experiment data is still available.

Value to start for log parameters

Choose the starting value for parameters displayed in the log scale from the drop-down list.