Normally, FCS Express imports the compensation matrix that was used when acquiring the sample. But sometimes you want to make your own compensation matrix.

There are two ways to create your own compensation definition in FCS Express:

 

•The Manual method allows you to adjust the compensation levels using sliders that allow you to adjust the amount of compensation.

•The Automatic method allows you to take single stained controls and automatically calculate the appropriate compensation values.

 

FCS Express allows you to quickly and easily set up either compensation method.  Sometimes compensation may also introduce digitization errors which will be discussed in the Compensation Digitization Error tutorial. In the following examples, we will be opening two layouts in FCS Express which already have a gate defined on the compensation population of interest.

 

The first example will show Manual Compensation.