In this tutorial, you will learn how to use MultiCycle to analyze different types of cell cycle histograms. There are ten different histogram files named ASCII1.fcs through ASCII10.fcs, located in the FCS Express Sample Data folder, that show different DNA cell cycles to explore MultiCycle.

The true strength of MultiCycle is its ability to analyze cell cycle histograms with multiple models coupled with the batch processing ability of FCS Express.

FCS Express includes a basic version of MultiCycle. The basic version will fit a single cycle model to your data without background or aggregate/debris correction. A single cycle analysis means only one population of cells will be modeled. The advanced version can fit up to two aneuploid populations (considered a 3-cycle model) as well as apply sophisticated background and aggregate/debris correction. The advanced version must be purchased separately.

In the course of this example, we will:

•Use gating to exclude doublets.

•Use Autofit in MultiCycle to analyze a DNA histogram.

•Format MultiCycle histograms.

•Use MultiCycle without Autofit to analyze a DNA histogram.

 

In the first exercise, we will use autofit selection for a single cycle analysis.