General Options

Option 

Explanation

Show Full Path

Under some circumstances, file names are displayed in FCS Express. This option allows you to choose whether the entire directory path and file name are displayed or only the file name.

Math Range Checking

When performing parameter math, it is possible for the result of a mathematical operation to exceed the allowed range of the file. Storing out-of-range values may cause improper results to be displayed when using data analysis packages other than FCS Express. If Math Range Checking is on, values greater than the maximum allowed value will be stored as the maximum allowed value. If Math Range Checking is off, the real out-of-range value will be stored.

When dragging a data set onto a plot

When dragging a dataset onto a plot, one of three possible actions will occur: replace existing overlays with the file being dragged onto the plot; add overlays to append the FCS file to the plot; or, prompt me to ask the user what action to take. To set the action, choose it from the drop-down list.

Default Compensation to Use

To set Compensation for new overlays, select one of the options below. See Selecting the Default Compensation for additional details:

•None. No compensation will be used.

•Compensation Default. The Compensation Default is selected in the Compensation Info window.

•FCS File. The Compensation used for acquisition will be taken from the FCS file header.

•FCS file, then Compensation Default. FCS Express will load Compensation from the FCS file. If there is no Compensation stored in the FCS file, the Compensation Default from the Compensation Info window will be used.

•Compensation Default, then FCS file. FCS Express will load the compensation default from the compensations navigator by default. If a default compensation does not exist, the compensation associated with the FCS file will be used.

Suffix for Compensated Data

When analyzing compensated data, the text typed into this box will be used as a suffix to describe the compensated data.  This text can be viewed in the Title of a plot when using the keyword $FCSE_FILENAME or $FCSE_FILENAMENOPATH

In new layouts, remember parameters by

FCS Express can remember the arrangement of parameters in the plots displaying data by their Parameter Number (Parameter 1, Parameter 2, etc.) or by their Parameter Name (FITC-A, FL2-H, etc.).  The Parameter Name used by this option is the keyword (or the combination of keywords) specified in the Keyword To Use as Parameter Name option (i.e. Name, Stain, Name and Stain, Stain and Name).

This option must be set prior to building the layout.

Show Hints on layout

When this checkbox is enabled, a hint will appear showing the plot ID and names of the files currently displayed when the mouse is hovering over the plot.

Maintain aspect ratio of layout objects after Maximize

When this checkbox is enabled, the aspect ratio (height vs. width) of objects will be maintained.

Measurement Units to Use

Choose Inches, Centimeters, or Millimeters from the drop-down list.

Appearance Options

These options allow you to chose the border colors for selected objects and objects that are being edited.

Default Zoom

You can customize the default zoom for your layouts.  Also, you can choose to Apply after opening saved layouts by default.

Apply after opening saved layouts

Applies the chosen default zoom level to layouts that were originally saved with a different zoom level.

Border color for selected objects

Choose the color of the border displayed when an object is selected from the drop-down color pallet.

Border color for object being edited

Choose the color of the border displayed when an object is being edited from the drop-down color pallet.

Print black and white inverted for plots with black background

When this option is selected, when displaying plots with black backgrounds, those plots will print out white, rather than black.

Performance Options

These options allow you to define preferences that may increase the performance speed of FCS Express.

When moving gates, quadrants, and markers, update plots statistics and tokens immediately

When checked, all gated events, backgating, and statistics associated with gates, quadrants, and markers will update immediately ("in real time") when gate, quadrant, or marker positions are changed.

Maximum Number of Undo Items

Sets the maximum number of undo items.

Maximum memory for cell images

Determines how many images stay in memory when picture plots are used. A higher value will increase performance until your computer's memory limit is reached.

Picture output options

These options allow you to choose the format, quality, and resolution of images of a plot copied to the clipboard for future use.

Use the screen resolution

Uses the resolution of the picture on the screen for an exported or copied picture.

Use this resolution

Uses a manually inputted value (in pixels) for the resolution of an exported or copied picture.

JPEG picture quality

Determines the quality of an exported or copied JPEG picture (Max of 100).

Duplicate object options

These options control where duplicated objects are placed after they are duplicated.

The position of the duplicated object is

Determines the position of a duplicated object relative to the original object using the drop-down list to define the position.

Distance between original and duplicated object(s)

Defines the space (in inches) between a duplicated object and the original object after duplication.

If duplicated object(s) are off the page, put them on a new page

If a duplicated object falls off of the page a new page will be created containing the duplicated object.