Kinetics Statistics
Contents
To display kinetics statistics in a default statistics table(Figure 27.4), right-click on the Kinetics plot and select Kinetics Statistics from the pop-up menu. Kinetics statistics can also be inserted as tokens anywhere on your layout.
Figure 27.4 Kinetics Statistics
The default kinetics statistics are explained in the table below. See Formatting Statistics to choose which statistics to display.
Statistic |
Explanation |
|---|---|
Overlay Number |
If there are multiple overlays visible on a plot, the statistics will be calculated for every overlay on the plot. Overlay # displays the overlay number for that row of statistics. The overlay number corresponds to the position in the overlay list on the Overlays page of the Format window. |
Overlay Legend |
The default legend is the file name. The legend can be changed in the Legend Text field of the Overlays page of the Format window. |
Gate |
The gate for which the statistics apply. This region is the 1D gate on the time parameter. |
Start Time |
The time at the left marker boundary, or the left boundary of the plot if there is no gate defined. |
End Time |
The time at the right marker boundary, or the right boundary of the plot if there is no gate defined.. |
Elapsed Time |
End Time minus Start Time. |
Peak Value |
The Y-axis value at the peak. |
Peak Time |
The X-axis value (Time) at the peak. |
Start to Peak Elapsed time |
Peak Time minus Start Time. |
Start to Peak Slope |
The slope from the left marker boundary to the peak, or from the left boundary of the plot to the peak. |
Start to End Slope |
The slope from the left marker boundary to the right marker boundary. |
Rising Phase Baseline |
What the algorithm determined was the baseline prior to the rising phase starting. |
Start Rising Phase |
The time at which the algorithm determined the rising phase started. |
Rise to Peak Elapsed Time |
Peak time minus Start Rising Phase. |
Rise to Peak Slope |
The slope from Start Rising Phase to Peak Time. |
Area Under the Curve |
Area under the curve in units of the Y-axis. |
Kinetics statistics can be calculated and displayed for the entire plot, or for gated events. Drawing gates on kinetics plots is advisable when the left boundary of the plot is not a reliable indicator of the baseline (e.g., when the signal is unsteady at the beginning of acquisition, or when a drug is added and the return to baseline is delayed). In such a case, the user may wish to define the baseline by creating a 1D gate on the Time parameter of the Kinetics plot. The left boundary of the 1D gate will define the Start Time statistic and the baseline, and the right boundary the End Time statistic.
The algorithm is designed to automatically detect the beginning of the rising phase and the peak.