Parameters Heat Map
Contents
The Parameters Heat Map plot type allows you to quickly compare parameters across gated populations and data files in a convenient visual format, without the requirement of having plots for each data file inserted on your layout. Unlike regular Plate Heat Maps, which only graph data files from multi-well plates, Parameters Heat Maps can graph data from all data files, regardless of whether they were acquired in plate format.
To insert a Parameters Heat Map plot, choose the Insert tab→ Other Plots→ Parameters Heat Map command, and click once on the layout where you want the plot to be inserted. Any default 2D-plot statistic for the parameters may be shown in a Parameter Heat Map. Below is an example where a statistic (Arithmetic Mean), from four different parameters ("FITC", "PE", "PE-Cy7", and "APC-Cy7"), is being compared across multiple data files and gates simultaneously, using the Parameters Heat Map plots at left and right, respectively (Figure 5.125).
Figure 5.124 Examples of Parameters Heat Maps (top) showing different parameters for multiple data files or gates, and the original plots on which the gates were drawn (bottom).
A Parameters Heat Map Stats Grid may be inserted for any Parameters Heat Map on the layout by right-clicking inside a Text Box, and selecting
Insert from the pop-up menu→ Insert Parameters Heat Map Stats Grid→ and selecting the Heat Map of interest (Figure 5.126).
Figure 5.125 Inserting a Parameters Heat Map Stats Grid inside a Text Box.
The Stats Grid contains the Statistic to show value for each cell that was chosen by the user in Gates Heat Map Overlays.
Many of the formatting features in regular Heat Maps are available for Parameters Heat Maps, and these are detailed in the following sections: