The statistics % Gated - Parent and % Gated - Grandparent are available in FCS Express.

See also % of custom denominator gate for 1D- and 2D plots.

 

These are defined as follows:

 

 

We will examine these statistics in terms of real data.  Figure 11.9 represents an analysis with sequential gating.  

 

 

When observing Gate 5...  In Plot #10 (the lower right 1D histogram), there is a Marker that was converted to Gate 5.  Gate 5 contains 40 events.

 

The gate that is applied to Plot #10 is Gate 4.  Therefore, the statistic for Gate 5 % Gated is defined as the number of Gate 5 events (40) divided by the number of Gate 4 events (2203) multiplied by 100.  In this case, the numerical formula is (40/2203)x100, which equals 1.8157.

 

The statistic for Gate 5 % Total is defined as the number of Gate 5 events (40) divided by the Total Number of events collected (20,000).  The numerical formula is (40/20000)x100, which equals 0.2.

 

The statistic for Gate 5 % of Gated - Parent is defined as the number of gated events (40) divided by the number of events for the parent of the gate applied to Plot #10.  As can be seen in Figure 11.10, Gate 4 has been applied to Plot #10.  The parent of Gate 4 is Gate 3.  So, the statistic for Gate 5 % of Gated - Parent is defined as the number of Gate 5 events divided by the number of Gate 3 events multiplied by 100.  The numerical formula is (40/2407)x100, which equals 1.6618.

 

 

The statistic for Gate 5 % of Gated - Grandparent is defined as the number of gated events (40) divided by the number of events for the grandparent of the gate applied to Plot #10.  As can be seen in Figure 2, Gate 4 has been applied to Plot #10.  The parent of Gate 4 is Gate 3.  The parent of Gate 3 is Gate 2.  So, the statistic for Gate 5 % of Gated - Grandparent is defined as the number of Gate 5 events divided by the number of Gate 2 events multiplied by 100.  The numerical formula is (40/2830)x100, which equals 1.4134.