FCS Express can generate any statistic that one would need for analysis of flow cytometry data. Statistics can be calculated either from a 1D or 2D plot. Statistics calculated base on a plot are only calculated for cells currently showing on the plot according to the current gate.

 

Statistics may be accessed in default statistics windows or tables or represented anywhere within FCS Express as tokens. Custom statistics may also be derived from any plot or user based input by accessing custom tokens.

 

FCS Express calculates statistics to be accurate to the IEEE single precision floating point standard, as described in http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=4610935&contentType=Standards and http://en.wikipedia.org/wiki/Single-precision_floating-point_format. Intermediate calculations may be performed with more precision, if available.

 

More information on the default statistics available for 1D- and 2D-plots, as well as using and formatting statistics are in the sections below:

•Histogram statistics

•2D plot statistics

•Custom tokens (user defined statistics and more)

•Using statistics and the clipboard

•Formatting statistics

 

 

The following newer statistics are described further in subsequent pages and are also available to be displayed as default statistics:

 

oPercent of Gated - Parent

oPercent of Gated - Grandparent

oBD Robust Standard Deviation (rSD in BD FACSDiva)

oBD Robust coefficient of variation (rCV in BD FACSDiva)