Manual Snapshots provide a way to save multiple gate, quadrant, or marker positions for quick access in your analysis. An unlimited number of manual snapshots may be created and saved in a layout. When a manual snapshot is added to the Manual Snapshots navigator using the blue + () button, FCS Express will remember the position of a any gates, quadrants, or markers present on open plots. When gates, quadrants, or markers are changed on open plots the saved manual snapshot may be applied to the layout by dragging and dropping the snapshot from the Manual Snapshots navigator to any blank space on the layout. The position of the gates, quadrants, and markers saved in the manual snapshot will be applied.

 

In the example below (Figure 7.36) note that the manual snapshot named "Gate Position Number 1" is applied to Sample 1 on the left. When data was changed to Sample 2 on the right, the position of the CD4 and CD25 gates was updated by applying (using drag and drop) the manual snapshot named "Gate Position Number 2". In this way many positions for gates, quadrants, or markers may be saved and easily accessed to be used as needed.

 

 

Please see the Snapshots chapter for more details on Snapshots.

 

Please see the Snapshots and Batch Iteration Navigation chapter for more details on using Snapshots with a batch process and the Next / Previous commands.

 

Also see the Snapshots Tutorial for step by step instructions on creating and using Snapshots.