Typically, FCS Express imports the compensation matrix that was used when acquiring the sample. However, you may need to make your own compensation matrix.

There are two ways to create your own compensation definition in FCS Express:

 

•The Automatic method allows you to take single stained controls and automatically calculate the appropriate compensation values.

•The Manual method allows you to adjust the compensation values manually, either through the Compensation Window or through sliders on plot.

 

Sometimes, compensation may also introduce digitization errors, which will be discussed in the Compensation Digitization Error tutorial. In the following examples, we will be opening two layouts in FCS Express, which already have a gate defined on the compensation population of interest.

 

The first example will show Manual Compensation.