FCS Express provides a complete DNA analysis solution based upon the widely used MultiCycle AV™ DNA analysis package written by Dr. Peter Rabinovitch at the University of Washington, and distributed by Phoenix Flow Systems (http://www.phoenixflow.com).

 

Multicycle AV™ is included free with each FCS Express license. Multicycle will allow users to fit up to 3 cell cycles with background, debris, and aggregate correction within a sample.

 

From the Multicycle manual:

 

"DNA content histograms require mathematical analysis in order to extract the underlying G1, S, and G2 phase distributions...The most flexible and accurate methods of cell cycle analysis are based upon building a mathematical model of the DNA content distribution, and then fitting this model to the data using curve-fitting methods." Multicycle contains "the most well established models," like those "proposed by Dean and Jett, based upon the prediction that the cell cycle histogram is a result of the Gaussian broadening of the theoretically perfect distribution," and Fox, whose "model provides a more flexible S phase shape, but still retains the smoothness of the S phase that is characteristic of the Dean and Jett model." In addition, "careful attention to fitting of the background aggregates and debris is also" a feature of Multicycle "in order to maximize the reliability of cell cycle analysis of complex histograms."