Layouts can be saved in any of the following ways:

 

•File tab→Save.

 

•Click the Save Current Layout icon in the Quick Access Toolbar.

 

•Use the Ctrl+S keyboard shortcut.

 

Using any of these methods will bring up the Save Layout dialog (Figure 4.2) where you can name the layout file to be saved and choose the location to save it.

 

 

When saving a layout, you have the option to save with a link, save without a link, or embed the data files within the saved layout according to the Save as type drop down option you choose.

 

• Layout Files (with linked data)(*.fey)

 

The position and formatting of the objects in the layout will be saved. When the file is opened, data will appear if the data files are available or have been copied to the computer where the layout is being opened. However, if the linked layout file is opened on a computer without access to the linked data files, no data will appear in the layout plots or statistics. Linked layout files are smaller than embedded layout files.

 

• Layout Files (with embedded data)(*.fey)

 

The position and formatting of the objects will be saved and a copy of the actual data files is saved within the layout file. This allows anyone who opens the layout file to see and analyze the data, without having the original data file. Although a layout file with embedded data files can be fairly large, it is actually only about two-thirds of the size of the original data file(s). This makes layouts with embedded files an ideal way to send data to a collaborator.

Note:  only .fcs files currently showing on the layout will be saved.  Files within the Data List but not currently showing on plots will not be included.

 

 

• Layout Files (with unlinked data)(*.fey)

 

The position and formatting of the objects (plots, statistics boxes, as well as the Data List options, including sorting) will be saved. No data will appear when the unlinked layout is opened. The file sizes of unlinked layout files are smaller than linked or embedded layout files.