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Spectrum Plot Specific Options

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Spectrum Plot Specific Options include Y Plot Resolution, Display Mode (Color on Gated/Overlays vs. Color on Density), Shade Based on Density, and Interpolate Values (Figure 5.60). For details on these options, please see the table in the Formatting a Spectrum Plot topic.

Figure 5.60 - Formatting Spectrum Plot Specific Options Dialog

These formatting options specific to Spectrum Plots are detailed in the table below. Standard formatting options for Spectrum Plot overlays are listed here.

Option	Explanation
Resolution	Increase or decrease the display resolution, or number of bins, of the Y-dimension of the plot.
Display Mode	The colors in Color on Density mode (left) reflect population density of events; the colors in Color on Gated/Overlays mode (center and right) reflect the following, depending on the number of overlays: •for plots with 1 overlay (center), the gate to which each event belongs (only for gates selected in the Display Gates Option) (e.g., pink for events in the "2D plot gate" below and the base color of the overlay (e.g., blue, below) for cells not in one of the selected gates). •for plots with >1 overlay (right), the overlay (e.g., red for Overlay 1, and green for Overlay 2).
Shade Based on Density	Superimposes a grayscale shading pattern based on the density of plotted data. When gates are displayed on a Spectrum Plot in the Color on Gated/Overlay Display Mode, the shading pattern will be pseudocolored to match the color of the gate displayed. A lighter shade represents a higher density. Note that this option will not apply if multiple overlays are displayed on the Spectrum Plot.
Interpolate values	FCS Express can interpolate values to impart a smoother look to your data, so that each event is depicted as a continuous line rather than an array of discrete values. Below are examples of plots with no interpolation (left), and 5 (center) and 50 (right) Interpolation Points between each value. Interpolate values is also applicable to Spectrum Plots with the Color on Density Display Mode: Please note that interpolation does impact the speed of displaying the plots. Thus, it is recommended to use the lowest interpolation value that provides the desired plot appearance.