ImageJ is free open-source software designed to process and analyze images. Through the use of macros and plugins users can automate tasks and create custom tools. De Novo Software has created macros and plugins for ImageJ that allow you to export many types of imaging experiments from ImageJ to FCS Express Version  (see our Example Webpage for specific examples). Once your data is properly exported from ImageJ, FCS Express Image Cytometry will allow you to perform a flow cytometry style data analysis on your data with all of our familiar 2D plots and new plotting features such as picture plots and heat maps.

 

The tutorial will use sample data to:

•Export a single image from ImageJ to FCS Express

•Create a .ijx Analysis Configuration file

•Use a .ijx Analysis Configuration file to export a group or set of images from ImageJ to FCS Express

 

For an overview and further descriptions regarding the De Novo Software ImageJ Macros please see the links below:

•General workflow for exporting ImageJ data to FCS Express

•ImageJ Macros for FCS Express General Overview

•Overview for the DNSMacroExample.ijm macro used to export data from ImageJ to FCS Express

•Overview for the DNSAnalysisCreator.ijm macro to create an .ijx Analysis Configuration File

•ImageJ for FCS Express Knowledge Base

 

Note: The tutorials will not focus on how to create macros or use ImageJ but will focus on the macros De Novo Software has provided. For more help with ImageJ please see the ImageJ website at http://rsbweb.nih.gov/ij/index.html or write to the very helpful and informative ImageJ Mailing List.