The DNA options refer to general, overlay, and position marker options for performing DNA analysis using Multicycle.

 

General

 

The General category of DNA Options (Figure 8.12) are miscellaneous properties dealing with the Multicycle fit model and the parameter to plot on DNA histograms.

 

 

Figure 8.12  DNA General Options

Figure 8.12  DNA General Options

 

 

The properties are described in the table below.

 

 

Option

Explanation

Fit Model to show

When Multicycle performs a fit, it calculates six different variations on that fit. You can choose which variation to display using this option. The six variations are described in the Multicycle manual.

Parameter Options

Select the parameter to display when changing data files. Use "Prompt when needed" to be prompted for the parameter every time you change a data file. The "Use parameter below" option always displays the parameter you set in the spin control.

 

 

Overlays

 

The Overlays category of DNA preferences (Figure 8.13) allows the user to set up how the calculated overlays look on the DNA histogram.

 

 

Figure 8.13  DNA Overlays Options

Figure 8.13  DNA Overlays Options

 

 

Select one or more overlays from the Select DNA Overlay list box on the left of the Overlays page to change the look and feel of that overlay. The visual properties that can be changed are similar to those for regular histogram overlays.

 

 

Position Markers

 

Position markers can be displayed on DNA histograms to mark the position of cell cycle components such as the G1 mean or the background region. The Position Markers category for DNA Options allows the user to set the default properties for these markers.

 

Select one or more categories from the list box on the left (Figure 8.14) and choose line options for the markers on the right.

 

 

Figure 8.14  DNA Position Markers Options

Figure 8.14  DNA Position Markers Options