Compensation/Unmixing Tokens
Contents
Compensation tokens allow you to insert information pertaining to the current compensation settings. You can choose different source and target parameters and display either the crossover or spillover matrix values (Figure 12.36). See Compensation and Spectral Unmixing for more information.
Gate Token |
Definition |
Spillover Matrix Value |
Represents the percentage of the signal from a given channel that spills into adjacent channels. |
Compensation Matrix Value |
Represents the inverse of the spillover matrix. Used to correct spillover. |
Unmixing Matrix Value |
Represents the estimate the contribution of each spectrum to the full spectra |
Spillover Spreading Matrix Value |
Represents fluorescence spectrum interactions that can limit the sensitivity of a reagent in the presence of brightly-stained cells on a different color. See Spillover Spreading Martrix |
Cross Stain Index Matrix Value |
Represent the spread of a given fluorochrome into another dye. See Cross Stain Index Matrix |
Figure 12.36 - Compensation Tokens
The following properties can be edited for Compensation/Unmixing tokens, by right-clicking on the token:
•Display Text - defines if the token should display the Token Value (default), Token Description, or a user-defined Text.
•Font - changes the font depending on the value of the token based on a list of classifications
•Number Format - allows users to select the number of decimals to be displayed, the Rounding mode and whether or not to show trailing zeroes if the value is numeric.
•The specific property for selected token, respectively:
oSpillover Matrix Value Details
oCompensation Matrix Value Details
oUnmixing Matrix Value Details
oSpillover Spreading Matrix Value Details
oCross Stain Index Matrix Value Details