The table below describes the Visualization pipeline steps that are available in FCS Express. If you would like to recommend additional Visualization methods to be provided with FCS Express, please contact support@denovosoftware.com.

 

 

Visualization

 

 

Step

 

 

Description

 

 

Graph Layout

 

Graph Layout allows you to change the visualization of your Minimum Spanning Tree (MST) on a Heat Map plot using a MST output as input parameter. Using the Filter: field a user can remove unwanted parameter options from view to simplify selection.

 

pipeline_graphlayout

 

Two different Graph Layout Methods can be selected: Arch (also called "U-shape") or Radio.

 

graph_layout_methods

 

Once the Graph is displayed on the Heat Map, the following actions can be performed on the Heat Map:

Modify the well size

Have well size dependent on a statistic such as number of events in the node

Set the Parameter and Statistic for display

Change the Color Level and Color Scheme

Create gate(s) to identify node(s) of interest, and edit other features

 

 

Minimum Spanning Tree

 

Creates a Minimum Spanning Tree (MST) using either a Cluster Assignment parameter or a Louvain Communities parameter as the input parameter. Please refer to the Clustering chapter for more details on how to generate Cluster Assignment parameters or Louvain Communities parameter.

 

Using the Filter: field a user can remove unwanted parameter options from view to simplify selection.

The MST step generates a new parameter that can be visualize on an Heat Map plot.

For more details on MST, please refer to this page on Wikipedia.

 

pipeline_MST

 

 

tSNE Layout

 

 

tSNE Layout allows you to change the visualization of your clustering parameter on a Heat Map plot using either a clustering assignment or a Minimum Spanning Tree as input parameter. The tSNE Layout organizes cluster nodes with on a tSNE map. tSNE coordinates for each cluster are calculated based on the location of each cluster in the multidimensional space.

Using the Filter: field a user can remove unwanted parameter options from view to simplify selection.

 

Please refer to the tSNE pipeline step for more details on the settings available to tune the tSNE algorithm.

 

In the example pipeline below, a tSNE Layout pipeline step has been used to map clusters generated by a Batch Self-Organizing Map clustering step.

 

tSNELayout_dialog

 

In the Heatmap below, an example of clusters mapped with a tSNE Layout is depicted.

 

tSNELayout

 

 

 

UMAP Layout

 

 

UMAP Layout allows you to change the visualization of your clustering parameter on a Heat Map plot using either a clustering assignment or a Minimum Spanning Tree as input parameter. The UMAP Layout organizes cluster nodes with on a UMAP map. UMAP coordinates for each cluster are calculated based on the location of each cluster in the multidimensional space.

Using the Filter: field a user can remove unwanted parameter options from view to simplify selection.

 

Please refer to the UMAP pipeline step for more details on the settings available to tune the tSNE algorithm.

 

In the example pipeline below, a UMAP Layout pipeline step has been used to map clusters generated by a Batch Self-Organizing Map clustering step.

UMAPLayout_dialog

In the Heatmap below, an example of clusters mapped with a UMAP Layout is depicted.

UMAPLayout