Index sorting allows researchers to review the complete phenotype of every single event sorted into a plate and associate that event with all sorted and non sorted events. FCS Express allows for analysis and reporting of index sorting data using Plate Heat Maps along with the standard plot offerings. For index sort data, Heat maps will display the number of events (usually one) and parameter based phenotypic data for all sorted events. Wells may be gated on individually, or as groups, with the well gates backgated onto other plots to view the individually sorted cells in context of the entire acquired/sorted samples.  All plate heat map functionality is available for use with index sorting data.

 

 

FCS Express currently supports index sorting data files from the following instruments:

•Influx (BD Biosciences)

•Summit Software (Beckman Coulter)

•Diva (BD Biosciences)

•Chorus (BD Biosciences)