Data Specific Gates in FCS Express allow you the ability to assign gates to specific data files that have been loaded in the data list.  Different gating positions between data files may be stored and automatically used when displaying plots or making calculations when using the Data Specific Gate feature, Figure 7.12.

 

 

 

When a gate is converted to a Data Specific Gates for a given file, the shape and position can be modified for the specific file independently from other data files. Thus, when a Data Specific Gates is created, the original gate (here hence referred to as "Master gate") continues to be used for all the remaining files.

 

Jump to:

•Creating Data Specific Gates

•Deleting/Removing Data Specific Gates

•Promoting Data Specific Gates to Master Gate

•Using Data Specific Gate Groups

 

 

An example of Data Specific Gates is given in Figure 7.13, where Gate 1 has been converted to be a Data Specific Gates for File 4. Files 1 through 3 continue to use the original position for Gate 1 (i.e. the Master Gate).

 

 

 

Data Specific Gates can be displayed/hidden in the Gate Navigator (Figure 7.14) and/or the Gate View. Please refer to Gate Navigator and the Gate View chapters to learn how to format those navigators in order to display/hide Data Specific Gates in them.

 

 

 

 

Although Data Specific Gates may appear to share some similarities with Iteration Snapshots and Manual Snapshots the features are quite different in term of their applicability:

 

•Data Specific Gates are specific for a data file in the data list, while Snapshot are specific for a given iteration number in the data list. Data Specific Gates are suitable for analyzing multiple FCS files simultaneously open in the layout, as well as multiple FCS file using batch process while Snapshots are suitable for analyzing multiple FCS files using the batch process.

•Data Specific Gates only account for gates and gating positions while Snapshots are used to record marker and quadrant positions along with gating positions.