The FCS File Options category (Figure 20.55, upper panels) allows you to select how FCS Files will appear in data selection dialogs (e.g., when changing files, adding files to the Data List, or inserting new plots). The FCS Express User Options are described in the table below.

 

Figure 20.54   FCS File Options dialog

Figure 20.54   FCS File Options dialog

 

 

 

 

Users may also define their own Instrument Specific Settings, which can be used to set the default Name and Stain that appears on plot axis, keyword to use for parameter matching, axis range, scaling, and division factors for scatter and fluorescence parameters on an instrument-specific basis.    

 

Option

Explanation

Dataset Name Keyword

Select the keyword to use as the default data set name when selecting from multiple data sets. Allow Multiple Data sets must be checked.

Allow Multiple Data sets

Some FCS files contain multiple data sets. If this option is checked, you can select one of the embedded data sets.  The user option to use Advanced Open Data Dialog also must be enabled to be prompted to select which data set upon loading the data files.

Default data set to use

This option specifies which data set should be loaded by default when Allow Multiple Data sets is not checked. In that case, when you select a file in the dialog that has multiple data sets, will load the data set number corresponding to the value selected instead of displaying a list of data sets. If the data set does not exist, FCS Express will load the first data set in the file.

FCS v3.0 $TIMESTEP units

Choose a unit to use when displaying a Time parameter in FCS Express from the drop-down list. The Default time option leaves the time parameter at the same raw value as is stored in the FCS file. Choosing a unit from the drop-down list will use the $TIMESTEP keyword to convert the raw value to the specified unit from the list.

Allow opening BD FACSDiva data files that were generated by exporting a FACSDiva Experiment

Check this box to allow opening data files that were exported from BD FACSDiva together with the experiment. See https://denovosoftware.com/faq/kb-why-do-i-get-the-message-that-a-data-file-exported-from-a-facsdiva-experiment-is-invalid/ for a detailed explanation. Re-exporting the files is a better option if you have access to FACSDiva and the experiment data is still available.

Plate Folder Import Options

These options allow users to automatically create the File Identifier (i.e. numeric) and/or the Classification (i.e. file names) Columns when FCS files are loaded as an FCS Plate Folder (see the Working with Heat Map chapter of the manual for more details on the FCS Plate Folder format).

An additional Classification Keyword can also be added using any of the keywords available in the loaded files. If a keyword is specified and multiple files share the same value for that keyword, events from those files will be grouped together in the same well.

 

 

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