Gates may be created on and applied to Spectrum Plots, much like any other plot type in FCS Express.  

 

Gating and backgating each work bidirectionally between Spectrum Plots and other plot types.  In other words, gates can be:

•created on Spectrum Plots and applied to / backgated onto other plot types (Figure 5 below Top)

•created on other plot types and applied to / backgated onto Spectrum Plots (Figure 5 below Bottom)