The Tools tab (Figure 3.28) contains the following groups of commands:

 

 

•Calculate

 

oThe Concentration Calculator command opens the Concentration Calculator window where you can calculate the concentration of events in a sample.

 

oThe Channel Calibration command opens the Channel Calibration window where you can change the axis fluorescence intensity scales from a linear or logarithmic scale to one indication either the absolute number of antigen molecules or the number of binding sites on the surface of a cell.

 

 

•Labels

 

oThe Parameter Labels opens a gallery containing Modify Labels, Load Labels, and Save Labels commands. These items allow the user to easily create, change, recall, and save alternate parameter labels.

 

 

•Snapshots

 

oThe Apply Iteration Snapshot command allows you to move gates between iterations and have the gates' new positions remembered while navigating using the Next and Previous commands.

 

oThe Snapshots command opens the Snapshot Navigator window where you can take custom snapshots of your analysis on data files and apply that analysis to other files.  For example, a snapshot of a gate position may be taken, the gate position changed, and snapshot applied to go back to the original gate position.

 

•Transformations

 

oThe Compensation and Unmixing command opens the Compensation and Unmixing Navigator, which allows you to display the compensation and unmixing information on the selected file, create new compensation and unmixing, and load or save compensation or unmixing information.

 

oThe Transformations command opens the Transformations Navigator, which allows you access K means (cluster analysis), Principal Component Analysis, Parameter Math (defines a single or sequence of math functions, save the functions, and apply the functions to data files), R integration to add new parameters or transformations, and tSNE. 

 

•Override

 

oThe Override Authorizations command allows you to override any Authorizations set on objects. See the Overriding Authorizations topic for additional details.

 

•SOP

 

oThe Run SOP command begins the step wise process of running through all of the steps defined in the SOP creation process

 

oThe Standard Operating Procedures command opens the Standard Operating Procedures navigator.

 

oThe Permission Rules command opens the Permission Rules Navigator where you can set available permissions specific to a layout.