Once a PCA transformation has been defined, it may be applied to any plot in FCS Express. PCA transformations may be applied to a plot by either of the following methods.  When a PCA transformation has been applied, the text "PCA transformed" will appear in the plot title.

 

Drag and drop a PCA from the Compensations window to the intended plot (Figure 16.29).

 

Figure 16.29 Applying a PCA transformation to a plot by dragging the PCA transformation from the Compensations navigator to the plot (green arrow).

Figure 16.29 Applying a PCA transformation to a plot by dragging the PCA transformation from the Compensations navigator to the plot (green arrow).

 

 

Access the Formatting Overlays dialog and choose the PCA to apply from the Transformation dropdown menu.

 

Figure 16.30 Applying a PCA transformation to a plot by selecting the PCA from the Formatting Overlays dialog in the Transformation dropdown.

Figure 16.30 Applying a PCA transformation to a plot by selecting the PCA from the Formatting Overlays dialog in the Transformation dropdown.