Once a PCA transformation has been defined, it may be applied to any plot in FCS Express. PCA transformations may be applied to a plot by either of the following methods. When a PCA transformation has been applied, the text "PCA transformed" will appear in the plot title.
• | Drag and drop a PCA from the Compensations window to the intended plot (Figure 16.29). |
Figure 16.29 Applying a PCA transformation to a plot by dragging the PCA transformation from the Compensations navigator to the plot (green arrow).
• | Access the Formatting Overlays dialog and choose the PCA to apply from the Transformation dropdown menu. |
Figure 16.30 Applying a PCA transformation to a plot by selecting the PCA from the Formatting Overlays dialog in the Transformation dropdown.