FCS Express 5 has the following new gating, marker, and quadrant features:

 

Gates / Quadrants / Markers:

Live updating gating and statistics.
Added ability to automatically create a marker gate for each marker.
Marker and gate text boxes can now be set to be unselectable.
Pasting a gate copied from a plot with different parameters is now allowed.
Ability to specify decimals for Marker endpoints.
New gates are created hierarchically by default.
A parent gate may now be selected when creating a new gate.
Enabled emphasizing gates on color dot plots and emphasizing low frequency populations.
Ability to specify font and color for quadrant text boxes.
Token for gate vertices.
Token for quad vertices.