Plate Heat Map Specific Options may be accessed by selecting the Plate Heat Map, and either:
•Select the Format tab→Plot Options→Specific command from the ribbon bar (Figure 5.123), or
•Right-click on the Plate Heat Map, select Format from the pop-up menu, then choose Specific Options from the dropdown.
Figure 5.121 The Plate Heat Map Specific Options dialog when accessed via the ribbon bar.
Plate Heat Map Specific Options allow to modify two Plate Heat Map properties:
•Orientation. This option allow to display the Plate Heat Map with Landscape or Portrait orientation. For Plate Heat Maps displaying SPADE, do not change this setting.
•Display space between the wells. The option allows you to remove any spacing between wells.
•Number of columns to display. This option applies to Plate Heat Maps that display Cluster Assignments from a k-Means transformation and for plate heat maps that are displaying merged files. It allows you to specify how many columns to display for Clustering Assignment rather than choosing a default number of well columns to display. For merged data files it determines how many columns wide the plate heat map should represent before "wrapping" to the next row. For instance, if 96 samples are merged together that represent a plate heat map, setting the value to 12 would result in a plate heat map with 12 columns and 8 rows.
•Node Size Range. This option allows to customize how the well size is calculated. When no Radius Statistics is defined in the Formatting Overlay Options, only the Max Size parameter is used. When a Radius Statistic statistics is defined in the Formatting Overlay Options both he Min Size and the Max Size are used. In both cases, the percentages define how smaller/larger will be the Min/Max well size as compared to the default setting (i.e. 200% means that the well will be twice as large as the default size, while 50% means that well size will be half of the default size). Some examples are depicted below in Figure 5.124.
Note: Setting the Node Size Range may be of particular use with many of the FCS Express pipelines, including FlowSOM.