Once overlays have been added to a Plate Heat Map (please refer to the Creating Plate Heat Map Overlays chapter), they may be edited similarly to Histogram Overlays and 2D Plot Overlays.

 

Formatting Options for Heat Map overlays can be accessed via the Formatting Options dialog. The Formatting Options dialog can be accessed by either:

 

1.Selecting one or multiple Heat Map plot(s) and then using the appropriate commands from the FormatObject Options group in the ribbon

2.Double clicking on the Heat Map plot.

3.Right-click on a Heat Map and choose Format from the pop-up menu.

 

The Overlays option controls the parameter, statistics to display, compensations, transformations, gates, and appearance of plate heat map overlays (Figure 5.110).

 

 

 

 

 

Figure 5.110 - Editing a Plate Heat Map Overlay

Figure 5.110 - Editing a Plate Heat Map Overlay

 

 

 

In the dialog, a list of all the overlays on the plate heat map will appear in the list box on the top. You can select one or more overlays using standard Microsoft Windows techniques, including Shift-Click and Ctrl-Click. The properties on the bottom pertain to the currently selected overlay(s). If multiple overlays are selected, the information will appear blank for those properties that are not identical in all selected overlays. If the properties are not blank, then they are identical for all selected overlays. Changing an option will change that property for all selected overlays. For instance, if you select three overlays and change the Gate, the Gate for all three overlays will be modified.

 

To remove an overlay from a plot, select the overlay (or multiple overlays) and press the Remove button.

To change the order of selected overlay(s) in the legend, use the up and down arrows (ReorderOverlays_grayArrows).

 

Plate Heat Map Overlay Options are explained in the table below.

 

 

Option

Explanation

Location parameter

Is the X parameter (well parameter or classification parameter) displayed on the plate heat map.

Statistic parameter

A drop-down menu to select the parameter from which a statistic will be chosen to be displayed. Please refer to the Choosing Parameters and Statistics chapter for more details.

Statistic to show

A drop-down menu to select the statistic to show as an overlay on the plate heat map. Please refer to the Choosing Parameters and Statistics chapter for more details.

Compensation

If compensation definitions are set, select the definition to use when compensating the data in the overlay.

Transformation

If transformations are applied to the data set, select the transformation to use when transforming the data in the overlay.

Gate

If gates are defined, select the gate that will be used to limit the data that is displayed on the histogram.

Radius Parameter

Optional: A drop-down menu to select the parameter from which a Radius statistic will be chosen. A Radius parameter must be chosen to use a Radius statistic. An example of Plate Heat Map using this option, is depicted at the end of the Choosing Parameters and Statistics chapter.

Radius Statistic

Optional: A drop-down menu to select the statistic to adjust the radius of each well.

Legend Text

The text that will appear in the legend beside the symbol for the overlay. If this field is blank the file name of the overlay will appear. Insert keywords from the header of the data file by pressing the ellipsis next to the legend text. You can also display the current gate applied to the overlay by typing "<$FCSE_GATEFORMULA>" into the legend text.

Change data file during Next/Prev/Batch

If this option is checked, the data file for this overlay will change when doing batch processing, or selecting Previous File or Next File. This option is usually only disabled for a control histogram, where you have multiple overlays, and want the control to stay the same all of the time.

Visible

You can hide overlays on a heat map without removing them. Select the overlay file from the list and uncheck Visible. Check Visible to show the overlay again.