Pipeline Steps

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Pipeline Steps

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Contents

The pipeline steps outlined in the table below are available in FCS Express. Please click on a step link or category to jump to a description and information on the variables accessible in the step.

Pipeline Step Table of Contents:

Category	Step
Pre-Defined Algorithms	FlowAI FlowCut FlowSOM SPADE
Downsampling	Gate Downsampling Interval Downsampling Mask Downsampling Random Downsampling Target Density Downsampling Weighted Density Downsampling
Dimensionality Reduction	PCA tSNE UMAP
Clustering	Batch Self-Organizing Map Consensus Clustering Hierarchical Clustering Kmeans Self-Organizing Map
Visualization	Graph layout Minimum Spanning Tree tSNE Layout UMAP Layout
Mathematical	0 to 1 Scaling Normalization Scaling Simple Parameter Math Thresholding
Quality Controls	Dynamic Range Downsampling Flow Rate Check Downsampling Low Time Density Downsampling Signal Acquisition Downsampling
Random Parameters	Random Parameters
Feature Extraction	Binned Variance Feature Extraction Variance Feature Extraction
Miscellaneous	Folder Jaccard Similarity Parameter K-Nearest Neighbors Parameter Merge to Spectra Parameter Removal Python Transformation Ramp Parameter Unmixing Virtual bandpass

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