The pipeline steps outlined in the table below are available in FCS Express. Please click on a step link or category to jump to a description and information on the variables accessible in the step.

 

 

Pipeline Step Table of Contents:

 

Category

Step

Pre-Defined Algorithms

 

FlowAI

 

FlowCut

 

FlowSOM

 

SPADE

 

Downsampling

 

Gate Downsampling

 

Interval Downsampling

 

Mask Downsampling

 

Random Downsampling

 

Target Density Downsampling

 

Weighted Density Downsampling

 

Dimensionality Reduction

 

PCA

 

tSNE

 

UMAP

 

Clustering

 

Batch Self-Organizing Map

 

Consensus Clustering

 

Hierarchical Clustering

 

Kmeans

 

Self-Organizing Map

 

Visualization

 

Graph layout

 

Minimum Spanning Tree

 

tSNE Layout

 

UMAP Layout

 

Mathematical

 

0 to 1 Scaling

 

Normalization

 

Scaling

 

Simple Parameter Math

 

Thresholding

 

 

 

Quality Controls

 

Dynamic Range Downsampling

 

Flow Rate Check Downsampling

 

Low Time Density Downsampling

 

Signal Acquisition Downsampling

 

Random Parameters

 

Random Parameters

 

Miscellaneous

 

Folder

 

Merge to Spectra

 

Parameter Removal

 

Python Transformation

 

Ramp Parameter

 

Unmixing

 

Virtual bandpass