You can set a gate on one or more wells in a plate heat map.  The gates can be created on manually selected well(s) or automatically on each single well within the plate or a selection of the plate.

 

To create a gate on manually selected well(s), the wells need not be contiguous.  To create a Well gate:

 

1. Select the Gating tabCreate GatesWell command. (Alternatively, right-click on the plate heat map and choose Create gateWell).

2. Click anywhere on the plate heat map. An Editing New Well Gate dialog (with instructions) for the well gate will appear above the plate heat map (Figure 5.87).

 

 

Figure 5.85  Setting a Well Gate

Figure 5.85  Setting a Well Gate

 

To set the specific gate do one or more of the following:

 

Click on an individual well to select it as part of the gate.

While holding the Ctrl key, click on multiple wells.

Click and drag a rectangle across multiple wells to select all wells within the rectangle as part of the gate.

 

The Create New Gate dialog will appear. Choose a name and color, and click on OK.

 

The well(s) in the gate will be outlined by a circle that is the same as the gate color (e.g., the wells outlined in light blue in Figure 5.88).

 

 

Figure 5.86 - Well Gate. Three wells are outlined in blue (rows E-G, column 03)

Figure 5.86 - Well Gate. Three wells are outlined in blue (rows E-G, column 03)

 

To change the gate you just created, click or click-drag on new well(s). The original well(s) will no longer be part of the gate.

 

To extend the gate you just created to additional wells, hold down the Ctrl key while clicking on individual wells (or while dragging a rectangle across a group of cells). The new well(s) will be added to the original well(s) in the gate.  Notice a non-contiguous region was selected (Figure 5.89).

 

 

Figure 5.87  Well Gates. Four new wells (rows E-F, columns 09-10) have been added to the previous gate by holding down the Ctrl key when dragging across the new wells.

Figure 5.87  Well Gates. Four new wells (rows E-F, columns 09-10) have been added to the previous gate by holding down the Ctrl key when dragging across the new wells.

 

 

Click Close when you are finished editing.

 

To create gates automatically on each single well (or cluster, if a cluster assignment is displayed instead of a well ID) within the plate or a selection of the plate

 

1. Select the Gating tabCreate GatesWell command. (Alternatively, right-click on the plate heat map and choose Create gateWell).

2. Click-drag the mouse around the selection of wells/clusters or all the wells in the plate for which single well gates are to be created. A an example, wells A01-B12 were selected in Figure 5.90 while 25 clusters have been selected in Figure 5.91.

3. Check the Create individual gates for each well or the Create individual gates for each cluster box (number1 in the figures below) and click OK (number2 in the figures below). The automatically created single well/cluster gates will appear in both the Gate View and the Gate Navigator.

 

Figure 5.88  Automatically creating single Gates on each well within a selection of the plate.

Figure 5.88  Automatically creating single Gates on each well within a selection of the plate.

 

 

 

 

Figure 5.89  Automatically creating single Gates on each cluster within a selection of the plate.

Figure 5.89  Automatically creating single Gates on each cluster within a selection of the plate.

 

 

 

 

To activate (and modify) an existing Well gate, right-click on the Plate Heat Map, select Edit gate, and choose the gate to activate from the submenu.