使用SPADE转换

一旦SPADE 被预设好,它可以被应用于FCS Express中的Heat Maps(热图)。请注意当为一个非多孔板数据插入一个热图时,将会出现一条警告信息(图2967)。

 

Figure 29.70 Warning message when inserting a heat map for data sets not derived from a plate based data file. 

Figure 29.70 Warning message when inserting a heat map for data sets not derived from a plate based data file. 

 

点击Continue(继续)并且通过如下几种方式按程序来应用SPADE转化:

 

 

从转化导航器上拖放SPADE到目标热图(图2968)。

 

Figure 29.71 Drag and drop SPADE from Transformations navigator to empty heat map. When the transformation has been applied the heat map will populate with SPADE nodes.

Figure 29.71 Drag and drop SPADE from Transformations navigator to empty heat map. When the transformation has been applied the heat map will populate with SPADE nodes.

 

进入Formatting Overlays Dialog (格式化叠加图层对话框)并在Transformation(转换)下拉菜单中选择并应用SPADE转换(图2969)。

 

Heat Map Options

 

以上2种方式的结果是出现一个热图,热图的m标题中会表示这是一个SPADE 树如下图所示,外轮廓是红色的(图2970)。

 

Figure 29.72 Heat Map with title appended with SPADE tree, outlined in red.

Figure 29.72 Heat Map with title appended with SPADE tree, outlined in red.

 

 

一旦SPADE被应用了,热图可以被格式化为:

根据例如节点的events的数目等统计数据调整为合适的大小。
设置展示的参数和统计
更改颜色的水平和配色方案
创建门来区分感兴趣的节点,并编辑其它的特征