Layout and Layout Performance Options

It is possible to access the Layout Options and Layout Performance Options by selecting the File tab→Info→Layout Options.

If you have security enabled, the Layout Group Security Access category will also be available.


The Layout Options category (Figure 4.17):



Figure 4.17  Layout Options Dialog

Figure 4.17  Layout Options Dialog


The specific options are described in the table below.




Layout Options

Displays information about the layout.

File Name

File path for the layout.


User Name of layout creator.

Date Created

Date layout was created.

Parameters remembered by

Indicates how parameters were set to be remembered in the User Options when the layout was created.  

Remember the scale formula for manually scaled plot parameters in this layout

When checked, allows you to remember the scale formula for manually scaled plot parameters. When the axis scale is changed to manual for one specific parameter on a plot, all other plots will then use the new scaling by default.

Requires Password

When checked, allows you to assign a password to open the layout.

Note: Use caution when deciding to password-protect a layout. If you forget the password, you will not be able to open the layout.

In this layout, use the following text for token errors

Allows you to define which text to use for token errors. Possible choices are: "#" or "##ERROR##" (both without quotes).

Startup Script

Customize the layout startup via the Startup Script tool.

Compensation Options

These options allow you to chose how compensation is applied to the current layout and whether to append or overwrite compensated parameters.

Suffix for Compensated Data

When analyzing compensated data, the text typed into this box will be used as a suffix to describe the compensated data.  This text can be viewed in the Title of a plot when using the keyword $FCSE_FILENAME or $FCSE_FILENAMENOPATH.

Suffix for New Parameters

When the option to append new compensated parameters to data after uncompensated parameters has been selected, the text typed into this box will be used as a suffix to identify the new compensated parameters from the uncompensated parameters.

New Compensated Parameters Should

When applying compensation to data, FCS Express allows the new compensated parameters to overwrite or be appended after the original uncompensated parameters. Note these options can be changed from the Compensation Options in the Compensation and Unmixing editor as well.

Overwrite uncompensated parameters. The new compensated parameters will appear in place of the uncompensated parameters. No suffix will be used to denote the new compensated parameters. The uncompensated parameters will not be available to plot.

Append to data after uncompensated parameters. The new compensated parameters will appear after the uncompensated parameters. The Suffix for New Parameters will be appended to each new compensated parameter name to identify the new parameter as having compensation applied.

Compensation/Unmixing to use in this layouts

Defines which compensation to use when loading files in the current layout. Select one of the options below. See Selecting the Default Compensation for additional details:

None. No compensation will be used.

Compensation/Unmixing Default. The Compensation/Unmixing Default is selected in the Compensation Info window.

FCS File. The Compensation used for acquisition will be taken from the FCS file header.

FCS file, then Compensation/Unmixing Default. FCS Express will load Compensation from the FCS file. If there is no Compensation stored in the FCS file, the Compensation/Unmixing Default from the Compensation Info window will be used.

Compensation/Unmixing Default, then FCS file. FCS Express will load the compensation/unmixing default from the compensations navigator by default. If a default compensation/unmixing does not exist, the compensation associated with the FCS file will be used. This is the default setting in FCS Express.



If you have security enabled, additional properties will be available on the Layout Options dialog.



The Layout Performance Options category (Figure 4.18) allows you to choose if live updating of gates, quadrants, markers, statistics and tokens is controlled at a global User Options level or at the Layout Level.


Choosing the Specify the value below radio button will allow you to turn the option to Update Immediately on or off for the current open layout via the check box.


Figure 4.18  Layout Performance Options Dialog

Figure 4.18  Layout Performance Options Dialog